Figure 1

Sensitivity of T315I mutation detection by AS-PCR method . Serial dilutions of T315I mutants with wild-type cells demonstrates 158-bp mutant bands in 100%, 25%, 10%, 1%, and 0.5% mixtures (Figure 1A); Representative samples of T315I mutated cell lines (Lane 1), T315I mutated CML case, (Lane 2), seven non-mutated non-leukemic cases (Lanes 3-9), and BA/F3 cell lines (Lane 10) are shown in Figure 1B; Lane 11, blank; Lane M, a 100-bp DNA marker.