Figure 6

SRSF1 plays an anti-apoptotic role in the response to chemotherapy. (A) The knock-down of SRSF1 resulted in an increase in early cell apoptosis. The percentages of annexin V(+)/PI (−) cells are indicated in the figure. sh-luc (targeting firefly luciferase) was used as a control. (B) The percentage of early apoptosis in each group is shown in the diagram. The knock-down of SRSF1 by sh-SRSF1-1 resulted in a higher percentage of early cell apoptosis. (C) Nalm-6 cells were treated with VCR, Ara-c or NS (negative control) at 48 hours after transfection with the shRNA plasmids. The cellular apoptosis was assessed at 72 hours after transfection by FACS analysis of annexin V-APC/PI staining. The percentages of annexin V(+)/PI (−) cells are indicated in the figure. (D) The diagram indicates the percentage of early apoptosis in each group. The knock-down of SRSF1 by sh-SRSF1-1 resulted in a further increase in early cell apoptosis induced by VCR or Ara-c.