Figure 1

Detection of tryptic peptides of rhSCF that contain the epitope recognized by the anti-rhSCF mAb. Reaction mixtures of tryptic digest of rhSCF were analyzed on SDS-PAGE and stained with Coomassie Blue (top) to visualize total protein, or on a Western blot (bottom) using the anti-rhSCF mAb (clone 23C8). Lanes 1–5, purified rhSCF digested with trypsin for 0.5, 1, 2, 4, and 6 h, respectively. The intact protein (A) and three proteolytic products (C-D) are indicated; D was not recognized by the anti-rhSCF mAb.