Figure 4

Effect of SNS-032, PP242, Rapamycin, and LY294002 on phosphorylation of mTORC1 and mTORC2 substrates in HL-60 cells. (A) HL-60 cells were treated with LY294002, Rapamycin, and PP242, respectively, at the indicated doses for 24 h. The anti-proliferative effects were measured by an MTT assay. The values are expressed as means ± SEM from three independent experiments. (B) HL-60 cells were treated for 6 h with LY294002 (25 μM), Rapamycine (10 nM), PP242 (2.5 μM), and SNS-032 at 50, 100, 200 and 400 nM, respectively. Whole cell lysates were then subjected to SDS-PAGE followed by immunoblotting with antibody that recognizes the corresponding antigen.