Figure 6

SNS-032 significantly reduces the expression of anti-apoptotic proteins in AML cells. (A) HL-60 and KG-1 cell lines were treated with SNS-032 at the indicated doses for 6 h. Cell lysates were fractionated on 6-12% SDS-polyacrylamide gels and analyzed by Western blotting with antibodies against xIAP, cIAP-1, Survivin, Mcl-1, Bcl-2 and actin. (B) AML cell lines were treated with SNS-032 at the indicated doses. After 6 h treatment, mRNA levels of the indicated genes were measured by real-time RT-PCR. The results are presented as the ratios of mRNA expression in treated cells in relation to the amount present in untreated cells (mean of ± s.d. mRNA from 3 different experiments). (C) HL-60 cells were treated with Rapamycin at the indicated doses for 6 h. Equal amounts of protein were resolved by SDS-PAGE and immunoblotted with the indicated antibodies.