Figure 2

Silvestrol down-regulates FLT3: (a) FLT3 mRNA expression in eIF4E immunoprecipitates from 50 nM silvestrol treated MV4-11 cells compared with that from untreated cells. Values are presented as mean ± SEM. (b) Expression of FLT3 examined by flow cytometry in MV4-11 and THP-1 cells after 24 hours exposure to 50 nM silvestrol. Blue = silvestrol-treated samples; Red = vehicle-treated control. (c) AML cells treated with 50 nM silvestrol and examined for FLT3 protein expression by immunoblotting. (d) MV4-11 cells treated with silvestrol or PKC412 (50 nM each) for 6 hours, and assessed for STAT5 phosphorylation by immunoblotting. (e) Primary AML cells incubated with increasing concentrations of silvestrol for 24 hours, and assessed for FLT3 protein expression by immunoblotting.