Figure 4

VEGF stimulated cords become insensitive to inhibitors of VEGF signaling in the ADSC/ECFC co-culture model. (a) Cords were established for 0, 1, 2, or 4 days, then treated with a concentration response of bevacizumab (Bev; left) or ramucirumab (Ram; right). Total tube areas for each were graphed. (b) Continuous monitoring of the effect of a concentration response of bevacizumab (Bev) on cord tube length beginning on day 0 (neoangiogenic mode; top) or after 4 days of establishment (established mode; bottom). (c) Continuous monitoring of the effect of a concentration response of ramucirumab (Ram) on cord tube length beginning on day 0 (neoangiogenic mode; top) or after 4 days of establishment (established mode; bottom).