Figure 4
From: TFPIα and TFPIβ are expressed at the surface of breast cancer cells and inhibit TF-FVIIa activity
PI-PLC and heparin treatment of HCAEC cells. Serum-starved cells were treated with SFM (untreated), PI-PLC (1 U/mL), or heparin (5U/mL) for 2 hours or with PI-PLC + heparin (2+2 hours) before the supernatant was removed and cells lysed or collected for flow cytometry analysis. The supernatant was discarded after PI-PLC pre-treatment. Total and free TFPI antigen measured in the supernatant (A and C) and cell lysate (B and D) of untreated/PI-PLC (A and B) and untreated/heparin/PI-PLC+heparin (C and D) treated HCAEC cells. Mean values (n ≥ 5; supernatant, n = 4; cell lysate) + SD of two independent experiments. Statistical comparisons were conducted between PI-PLC or heparin/PI-PLC+heparin treated samples and untreated controls. (E) Flow cytometry analysis of untreated (black, solid), PI-PLC (black, broken, left panel), and heparin (black, dotted, right panel) treated HCAEC cells. One representative experiment of three is shown.