Figure 6

Cell death induced by GBR401 is dependent on homotypic adhesion and on actin microfilament reorganization. Mec-1, Raji and SU-DHL-6 cells were pre-incubated with the inhibitor of actin polymerization latrunculin B and treated with mAbs (1 μg/mL) during 2 hours. A/ Homotypic adhesion was assessed by light microscopy (original magnification x20). Raji cells are shown as a representative example. B/ Cell death and absolute alive cell number were analyzed by annexinV/7AAD flow cytometry staining. C/ The clonogenic capacities of Raji and SU-DHL-6 cells were assessed with CFU assays. D/ Raji cells were sedimented onto microscope slides. After fixation with 4% paraformaldehyde in PBS, cells were permeabilized, stained with Phalloïdin-A488 and analyzed by fluorescent microscopy (scale bar: 10 μm). All the measurements were realized in triplicates and the graphs represent mean values +/- SD.