Figure 2
Methylation analysis in myeloid-derived cancer cell lines. (A) A detailed map of the WT1 promoter interval with the methylation profile for CD34+ and leukocyte cells determined by WGBS. Vertical dark grey lines in the WGBS tracks represent the mean methylation value for individual CpG dinucleotides calculated from multiple data sets, with the light grey lines representing the mean + standard deviation. The positions of the bisulphite PCR products are indicated. (B) The methylation status of the WT1 and AWT1 promoters and the WT1-ARR region in control leukocytes and immuno-selected cells. Each circle represents a single CpG dinucleotide on a DNA strand, a methylated cytosine (●) or an unmethylated cytosine (ο). Alrozygous samples. (C) The methylation profile in AML cell lines. (D) Quantitative PCR on meDIP preciptations using specific antibodies against 5hmC and 5mC in three cell lines that show hypermethylation of the AWT1 CpG island using bisulphite techniques.