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Figure 7 | Journal of Hematology & Oncology

Figure 7

From: The Src homology-2 protein Shb modulates focal adhesion kinase signaling in a BCR-ABL myeloproliferative disorder causing accelerated progression of disease

Figure 7

Effect of FAK inhibition on colony formation of wild-type and Shb knockout BCR-ABL-transformed bone marrow cells. c-Kit-enriched bone marrow cell (104) were plated in methylcellulose in the absence of cytokines in the absence or presence of 10 μM FAK inhibitor 14 and cultured for 5 days after which colony numbers were determined. (a) Colony formation of bone marrow cells isolated from wild-type recipients. (b) Inhibition of colony formation by inhibitor on bone marrow cells isolated from wild-type recipients. (c) Colony formation of bone marrow cells isolated from Shb knockout recipients. (d) Inhibition of colony formation by inhibitor on bone marrow cells isolated from Shb knockout recipients. The values in (b) and (d) were obtained by subtracting the inhibitor values from the corresponding control values. Means ± SEM for 6 dishes (three mice) each group are given. * and ** indicate p < 0.05 and 0.01, respectively with one way ANOVA (Bonferroni). # indicates p < 0.05 when compared with wild-type using a Students’ t-test. Ns = not statistically significant.

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