Figure 3

BENC-511 downregulates PI3K/AKT downstream signals. OPM2, RPMI-8226 and LP1 cells were treated with increasing concentrations of BENC-511 for 24 hours. Whole lysates were subjected to Western blot analysis. (A) p-mTOR (Ser2448), T-mTOR, Raptor; (B) p-p70S6K, p70S6K, p-4E-BP1, and 4E-BP1; (C) p-GSK-3β (Ser9). β-actin was used as an internal control.