Figure 2
From: DACH1 inhibits cyclin D1 expression, cellular proliferation and tumor growth of renal cancer cells
Epigenetic silencing of DACH1 enhanced cellular proliferation. A. Western blot analysis of DACH1 and cyclin D in embryo kidney cells and clear cancer cells. B. Relative DACH1 mRNA expression normalized to GAPDH with or without treatment. C. Protein abundance with or without treatment. β-tubulin as loading control. Cellular proliferation analyzed by MTT assay (D) and cell counting (E).