Figure 2

Molecular inhibition of c-FLIP induced T-lymphoma cell apoptosis and sensitized tumor cells to chemotherapeutic agents. A, c-FLIP expression of Jurkat and H9 cells transfected with Con siRNA or c-FLIP siRNA was confirmed by western blot (Left panel). Cell apoptosis of Jurkat and H9 cells transfected with Con siRNA or c-FLIP siRNA was measured by Annexin-V/PI assay (Right panel). B, TRAIL and DR5 expression of Jurkat and H9 cells transfected with Con siRNA or c-FLIP siRNA was assessed by flow cytometry. MFI, median fluorescence index. C, IC50 of doxorubicin, cyclophosphamide and cisplatin using Jurkat cells and H9 cells transfected with Con siRNA or c-FLIP siRNA were determined by MTT assay after 72 h incubation with chemotherapeutic agents. D, Cell apoptosis of Jurkat cells and H9 cells transfected with Con siRNA or c-FLIP siRNA was measured by Annexin-V/PI assay after treatment with doxorubicin (75 nm), cyclophosphamide (3 mM) and cisplatin (4 μM) for 72 h, respectively. *, P < 0.05, **, P < 0.01 comparing with the negative control (Con siRNA).