Figure 2

Galloflavin inhibited cell proliferation in primary cultures of endometrial cancer. Eight primary cell cultures of endometrial cancer were cultured in 96 well plates and treated with GF as indicated doses for 72 hours. Cell proliferation was assessed by MTT assay (A). LDHA protein expression in the primary cell cultures was detected by Western blotting (B). Eight established endometrial cancer and ovarian cancer cell lines were cultured in 96 well plates, and treated with GF for 72 hours. Cell proliferation was assessed by MTT assay (C). LDHA and c-Myc protein expression in the eight cell lines was detected by Western blotting (D). The relationship of LDHA and c-Myc protein expression and cell response to GF was assessed by Pearson correlation. The results showed a significant correlation between LDHA expression and cell growth inhibition by GF (Pearson r =0.77026; p = 0.0005; n = 16) (E). There was no significant correlation between c-Myc protein expression and cell sensitivity to GF (F) (* < 0.05).