Figure 1

Mantle cell lymphoma patients display a broad repertoire of specific T cells to cyclin D1. PBMCs were isolated from a MCL patient (ACC-2000, HLAA* 02010101*3201, B*1501*3503, C*0303*1203, DRB1*0401*1401, DQB1*0503*0302), then 1 × 10⁶ cells per sample were stimulated with 71 individual 15-mer cyclin D1 peptides from Table 2. Median plus 5 multiplied median absolutedeviation (MAD) is considered a positive cutting line (shown as a red dash line). (A) Supernatants were harvested to test IP-10 and IL-2 secretion after 48-h co-culture. NoP is a no peptide negative control. (B) Supernatants of PBMCsafter 48-­h boosting were harvested to test cytokine IL-­2 and IFN-­γ secretion by Luminex®. The cells without peptide were used as negative control. Percentage of CD4⁺IFN-γ⁺ and CD8⁺IFN-γ⁺ population from IFN-­γ intracellular staining (from (C) was shown in a two-­line graph. (C) CFSE-labeled PBMCs were stimulated with cyclin D1 for 8 days and rested in serum-free medium for 2 days before boosting by the same peptide. Intracellular staining of IFN-γ was performed 6 h later. The cells without peptide were used as a negative control.