Fig. 6

Angptl4 increases the number of CD61 + CD41^low/negative MKs in vitro. a Representative FACS profile of CD61⁺CD41⁺, CD61⁺CD41^low/negative, and CD61⁻CD41⁻ cells in SCF-, SCF + TPO-, SCF + Angptl4-, and SCF + TPO + Angptl4-supplemented cultures at day 5 after initiating cultures from 1 × 10⁵ Lin⁻ BM cells. b Total cells developed and total number of CD61⁺CD41^low/negative and CD61⁺CD41⁺ cells in cytokine-supplemented cultures as described in a. Mean ± SEM of three different experiments with a total of 12 SCF-; 12 SCF and TPO-; 12 SCF and Angptl4-; and 12 SCF, Anptl4, and TPO-supplemented cultures are shown. Statistically significant differences are indicated (*p < 0.05, **p < 0.01, ***p < 0.001). c Representative cytospin preparation and Wright-Giemsa staining of MK cultures as described in a. Original magnification ×400