Fig. 2

Effects of palmitoylation on transformation potential of oncogenic NRAS vs. KRAS4A in vitro. a The growth curves of Ba/F3 cells transduced with the MGI vector control, KRAS4A^G12D, KRAS4A^G12D/C180S, NRAS^G12D, or NRAS^G12D/C181S after removal of IL-3. Student t test was used for the statistical analysis of the cell numbers in triplicate at each time point. ****P < 0.0001. b Immunoblotting of lysates of NIH3T3 cells stably expressing the GFP control, KRAS4A^G12D, KRAS4A^G12D/C180S, NRAS^G12D, or NRAS^G12D/C181S using a pan anti-RAS antibody. The top band represents Myc-tagged-RAS; the bottom band represents endogenous RAS. c Morphology of cultured NIH3T3 cells stably expressing the indicated proteins was observed under light microscope (original magnification, ×200). d Quantification of anchorage-independent growth of NIH3T3 cells expressing the indicated proteins. Colonies were counted on day 14 after plating in triplicate. Student t test was used for the statistical analysis. **P < 0.01, ***P < 0.001