Fig. 2

Rheb1 deletion prolongs the survival of AML mice. a Experimental scheme of the Rheb1-deficient MLL-AF9 mouse model. b The survival curve of Rheb1 ^fl/fl and Rheb1 ^Δ/Δ AML mice using Kaplan-Meier analysis (n = 11). Approximately 5 × 10⁵ P1 GFP⁺ cells from the two groups were transplanted into sublethally irradiated recipient mice (P2). c The survival curve of Rheb1 ^fl/fl and Rheb1 ^Δ/Δ AML mice calculated using Kaplan-Meier analysis (n = 6). Approximately 1 × 10⁶ P2 GFP⁺ cells obtained from the two groups were transplanted into sublethally irradiated recipient mice (P3). d The number of white blood cells (WBC) in PB (n = 4). e The number of platelets (PLT) in PB (n = 4). PB samples were obtained from AML mice when Rheb1 ^fl/fl GFP⁺ cells in PB was approximately 80 %. f–h The percentage of GFP⁺ cells in the PB (f), BM (g), and spleen (h) of Rheb1 ^fl/fl and Rheb1 ^Δ/Δ AML mice by FACS analysis (n = 4). i The spleen size (left) and spleen weight to body weight ratio of Rheb1 ^fl/fl and Rheb1 ^Δ/Δ AML mice (right). All experiments were performed at least three times. The values in the panels represent the mean numbers ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001