Fig. 3

PU.1 transactivates HOTAIRM1 through the +1100 region in the HOTAIRM1 promoter. a Schematic representation of pGL3-HOTAIRM1 as well as its mutated and truncated forms. b PU.1-transactivated promoter activity of HOTAIRM1. Loss of major PU.1 binding sites significantly impaired PU.1 transactivation on the HOTAIRM1 promoter. The luciferase promoters were co-transfected with equal amount of the pcDNA3.1 or pcDNA3.1-PU.1 vector to HEK-293T cells. The data represent the mean of three replicates ± SD. *p < 0.05; **p < 0.01