Fig. 3

SOCS3 was up-regulated by imatinib. a The expression of SOCS3 in K562 and KU812 cells was examined by qPCR and western blotting after they were treated with imatinib for 48 h. β-Actin was served as an internal control. Each value was the mean ± SD of three experiments (n = 3), *P < 0.05; compared with untreated cells. b Apoptosis of K562 and KU812 cells, which were transduced with SOCS3 over-expression or empty vectors, were examined by Annexin V staining following treatment with imatinib (48 h). c Statistical analysis of apoptosis in K562 and KU812 cells after imatinib treatment. Data represented the mean ± SD from three experiments (n = 3). *P < 0.05; compared with cells transduced with empty vector