Fig. 8

B4GALT1 was regulated by SOCS3/miR-124-3p axis. a Western blot analysis of B4GALT1 expression in K562 cells after SOCS3 up- or down-regulation. β-Actin was served as an internal control (n = 3). b Western blot analysis of B4GALT1 expression in K562 cells stably expressing SOCS3 after transduction with the miR-124-3p inhibitor or negative control. β-Actin was served as an internal control (n = 3). c Over-expression of B4GALT1 rescued SOCS3-induced chemo-sensitivity promotion. B4GALT1 expression and control vector were transduced into K562 cells that over-expressed SOCS3. Apoptosis ratios of K562 cells in different groups were examined by Annexin V assay after imatinib treatment. Data represent the mean ± SD from three experiments (n = 3). d SOCS3 over-expression inhibited tumor growth of K562 in vivo. *P < 0.05; compared with empty group. Each point represented the mean of three independent experiments (n = 3). Data are expressed as the mean ± SD. e SOCS3 over-expression inhibited the expression of B4GALT1 in vivo. B4GALT1 expression in tumors from K562 cells was analyzed by immunohistochemistry