Fig. 1

Effect of MACC1 on the resistance to trastuzumab in HER2-positive GC cell lines. a Western blot analysis of MACC1 expression in NCI-N87 and MKN45 parental cells and trastuzumab-resistant cells NCI-N87/TR and MKN45/TR. GAPDH was used as a loading control. b Western blot analysis of protein extracts from NCI-N87/TR and MKN45/TR cells 48 h after transient transfected by siMACC1 or siCtrl. NC, NCI-N87/TR, or MKN45/TR cells. GAPDH was used as a loading control. c Percentage of cell viability. MTT assays were performed 72 h after trastuzumab (Ttzm) treatment of the indicated cells at the different concentrations. Data represent mean ± SD of triplicate experiments relative to untreated cells. *P < 0.05, ^# P < 0.01, ⁺ P < 0.001. d Percentage of cell viability. MTT assays were performed 72 h after trastuzumab treatment of the indicated cells at the different concentrations. Data represent mean ± SD of triplicate experiments,*P < 0.05, ^# P < 0.01, ⁺ P < 0.001