Fig. 6
From: DNMT3A mutation leads to leukemic extramedullary infiltration mediated by TWIST1
TWIST1 mediates enhanced cell migration ability in vivo. a Representative bioluminescent imaging of mice transplanted with OCI-AML3 cells carrying luciferases and stably expressing scramble shRNA (shControl) or shRNA targeting TWIST1 (shTWIST1). Quantification of bioluminescent imaging in brains and whole bodies is shown on the right panel. Values are presented as mean ± SEM (n = 6 per group). b Scatter plots present the OCI-AML3 cells with or without TWIST1 mRNA knockdown in murine BMs and brains, respectively. The percentage of exogenous cells is gated in blue box. c HE staining of BM and brain biopsies taken from shControl or shTWIST1 mice. Black arrows indicate leukemic cells in marrow cavities or brain meninges. d Immunohistochemical staining of hCD44+ cells indicated by red arrow in shControl murine brain. Black triangles denote cerebral parenchymas. Dashed lines indicate the meninges. e Kaplan-Meier analysis shows the survival rates of shControl and shTWIST1 mice (n = 6 per group)