Fig. 1
RR cells express a substantially higher level of MYC than RU cells. a The top 11 factors that are predicated to bind to SRR2 sequence by JASPAR motif matches analysis at P < 0.001. b The relative mRNA expression levels of putative SRR2 binding factors assessed by quantitative RT-PCR (qRT-PCR). GAPDH was used as internal control, and all the mRNA expression levels were normalized to that of Sox2 in RU cells. Note that the mRNA levels of POU5F1 and SOX17 in both cell lines were undetectable by qRT-PCR. c The protein levels of p-MYCS62 and MYC in RU and RR cells derived from SupM2 and Karpas 299. d The subcellular localization of p-MYCS62 and MYC in RU and RR cells derived from SupM2 and Karpas 299, assessed by the nuclear cytoplasmic fractionation assay