Fig. 2

Caspase-1 substrates and interaction proteins are present in cellular compartments other than the cytosol. a There are 114 newly experimentally verified caspase-1 substrates: 22 of these caspase-1 substrates are secretory proteins, 5 are plasma membrane proteins, 3 Golgi proteins, 7 ER proteins, 1 mitochondrial protein, 14 cytoskeleton proteins, 33 cytosolic proteins, 28 nuclear proteins, and 2 endosomal proteins. 28% of the identified caspase-1 substrates are present in the cytosol while the rest of the 72% of the substrates are generally localized elsewhere other than in the cytosol. 25% caspase-1 substrates are localized in the nucleus and 19% caspase-1 substrates are secreted extracellularly. b Out of 38 experimentally verified caspase-1 interaction proteins, 6 caspase-1 interaction proteins are secretory proteins, 7 are plasma membrane proteins, 1 Golgi protein, 3 ER proteins, 2 mitochondrial proteins, 2 cytoskeleton proteins, 18 cytosolic proteins, 11 nuclear proteins, and 1 endosomal protein. In addition to 35% cytosolic interaction proteins, caspase-1 has as high as 63% probabilities to traffic to other subcellular organelles to form protein interactions. 21% of caspase-1 interaction proteins are localized in the nucleus and 11% of caspase-1 interaction proteins are secreted extracellularly