Fig. 4

MAGL activated the NF-κB signal pathway in HCC cells. a, b The protein and mRNA expression of NF-κB p65 were evaluated in the indicated four HCC cell lines by western blot and qRT-PCR analysis. HepG2-MAGL cells were cultured with MAGL-inhibitor JZL184 (1 μM) for 0, 0.5, 1, and 4 h. c Western blot analysis revealed that the total protein level of NF-κB p65 was significantly downregulated in a time-dependent manner by the treatment of JZL184. d The phosphorylation of NF-κB p65 decreased in a time-dependent manner when treated with JZL184. e MAGL-inhibitor JZL184 could not inhibit the expression of NF-κB p65 in cytoplasm as culture time extended. f The expression of NF-kB p65 in the nucleus was significantly reduced in a time-dependent manner when treated with JZL184. Data, mean + SD. *P < 0.05, **P < 0.01, and ***P < 0.001