Fig. 2

DC-SIGNR promotes colon cancer cell migration and invasion in vitro. a Crystal violet staining showed that DC-SIGNR positively promoted the migration and invasion ability of the three colon cancer cell lines. Scale bars, 100 μm. b The in vitro migration and invasion promoting activities of colon cancer cells treated with the DC-SIGNR protein or control IgG were measured by determining the number of cells that passed through the Transwell membrane. c Morphology micrographs of the three types of colon cancer cells treated with the DC-SIGNR protein or control IgG for 24 h. The DC-SIGNR protein induced scattering of the three types of colon cancer cells. Scale bars, 50 μm. d Pictures of the wound healing assay in LoVo cells incubated with the DC-SIGNR protein or control IgG at 24 h after the cells were scratched. The LoVo cells treated with the DC-SIGNR protein migrated more quickly than those treated with control IgG. Scale bars, 100 μm. e The relative cell migrations of the three colon cancer cell lines at 24 h after the cells were scratched are shown in this panel. The error bars in all graphs represent SD, and the results were quantified from three experiments