Fig. 7

ENO1 silencing affects in vitro and in vivo cell migration and invasion. a Migration ability was evaluated in terms of capacity to close the wound of shENO1 CFPAC-1 cells compared to shCTRL control cells. Representative images are shown for each condition. b Invasive potential of shENO1 and shCTRL cells were tested through a Matrigel invasion assay, after 48 h of culture. For quantitative analysis, invasive cells were fixed, stained with crystal violet, treated with acetic acid and elutes were read at a wavelength of 570 nm. Results are expressed as OD. c Lung metastatic area was evaluated after 28 days from i.v. injection of shCTRL or shENO1 CFPAC-1 cells in the tail vein of NSG mice. The histogram represents the percentage of metastatic area compared to the total lung (left panel). Representative images of the lung are shown for each group of mice (right panels). For the in vivo experiments, five mice per group were used. A representative of three independent experiments is shown. Data are mean ± SEM. *p < 0.05 relative to control cells