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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Antitumor activity of miR-34a in peritoneal mesothelioma relies on c-MET and AXL inhibition: persistent activation of ERK and AKT signaling as a possible cytoprotective mechanism

Fig. 2

Effects of miR-34a reconstitution on DMPM cell proliferation, apoptosis, and RTK signaling pathways. a Antiproliferative effects of miR-34a. DMPM cell growth was assessed by cell counting. Data are expressed as percentage of the proliferation of miR-34a- versus miRNA negative control (Neg)-transfected cells. Means ± SD values of three independent experiments are reported. b Proapoptotic effects of miR-34a. Quantitative analysis of TUNEL-positive DMPM cells transfected with transfection reagent (Ctrl), Neg, or miR-34a was carried out by flow cytometry at different time points after transfection. Mean ± SD values of three independent experiments are reported (**p < 0.01; ***p < 0.001 by Student’s t test). c, d Effects of miR-34a on validated miRNA targets and RTK downstream signaling cascades as assessed by western blot analysis at 72, 96 (c) and 168 (d) h after cell transfection with Ctrl, Neg, or miR-34a. Actin was used to confirm equal protein loading. A representative experiment of three was reported. Cropped images of selected proteins are shown

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Journal of Hematology & Oncology

ISSN: 1756-8722

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