Fig. 6

miR-34a inhibits the invasion of DMPM cells. Cells were transfected with Ctrl, Neg, or miR-34a for 24 h. a Cells were subjected to Matrigel invasion assay in serum-free medium 72 h after transfection. Top: the number of invading cells per field is reported. Histogram bars represent mean values ± SD of three independent experiments (**p < 0.01; ***p < 0.001 by Student’s t test). Bottom: micrographs from one experiment representative of three. Original magnification, × 40. b Whole-cell lysates were analyzed by western blot with anti-phospho-FAK. Protein extraction was performed 96 h after transfection with Ctrl, Neg, or miR-34a. Vinculin was used to confirm equal protein loading. A representative experiment of three was reported. Cropped blots are shown