Fig. 2
TSP-2 is highly expressed in metastatic human PCa cells. a Total proteins were extracted from PZ-HPV7, DU145, and PC-3 cells and subjected to immunoblotting analysis using anti-MMP-2 and TSP-2 antibodies, and re-probed against β-actin to show equal loading amounts. b The MMP-2 (open bar) and TSP-2 (closed bar) mRNA expressions of PZ-HPV7, DU145, and PC-3 cells were examined by real-time PCR. c The migratory (open bar) and invasive (closed bar) abilities of PZ-HPV7, DU145, and PC-3 cells were determined by the transwell assay. d Total protein was extracted from PC-3, PC-3-I3, DU145, and DU145-I5 cells and subjected to immunoblotting analysis using anti-MMP-2 and TSP-2 antibodies. The MMP-2 and TSP-2 mRNA expression (f and h) as well as migration and invasion (e and g) ability of PC-3, PC-3-I3, DU145, and DU145-I5 cells were determined by RT-PCR and the transwell assay, respectively. Results are expressed as mean ± SEM. *p < 0.05 when compared to PZ-HPV-7, PC-3, or DU145 cells