Fig. 7

Knockdown of TSP-2 decreases cell migration and osteolytic metastasis in vivo. PC-3 constitutively expressed pLenti CMV V5-Luc cells were a transfected scramble of (PC-3/Luc) or TSP-2 shRNA (PC-3/shTSP-2-Luc), followed by determining their TSP-2 protein (a), or mRNA (b), and miR-376c (d) expressions by immunoblotting and real-time PCR, respectively. The migration (open bar), invasion (closed bar) (c), or cell viability (e) were also determined by the transwell assay and MTT analysis, respectively. f These cells were injected into the right tibia bone of nude mice and detection by bioluminescence imaging at the indicated time intervals, and (g) these images were quantified (photons/s of the right leg). After 4 weeks, these mice were sacrificed, and the tumors were excised (h) and their weights were determined (i). The expressions of TSP-2 and MMP-2 on these tumors were then assessed by immunoblotting (j) or IHC staining (k). l Represents the micro-CT images of the tibia from sacrifice mice. Scale bar = 1 mm. m This represents quantification of the resorption area of the tibia. WT wild type, KD knockdown