Fig. 2

Quantitative PCR of selected mediators of GLIS2 pathway a BMP2 and b GLIS2 after 48 h treatment with GANT61. *p < 0.05; **p < 0.01; ***p < 0.001. c Hierarchical clustering of genes differentially expressed between untreated and treated AML leukemia blast cells and cell lines, with a p value <0.05. d ChIP analysis, performed on WSU-AML cell line showed around five-fold enrichment of chimeric protein on DNMT1, DNMT3B, and NCAM1 promoters. e Western blot analysis showing the decrease of GLIS2 protein and CBFA2T3-GLIS chimeric protein in samples treated with GANT61. Thirty micrograms of protein were blotted to each lane. Antibody to β-actin served as a loading control. The Relative Induction (Rel Ind) is the amount of protein present in treated samples relative to untreated cells after normalizing to β-actin density