Fig. 4

Modulation of miR-130b and miR-301b induces cell senescence. Cell area (a) and sphericity (b) were increased upon miR-130b or miR-301b overexpression and compared to the pre-miR-NC transfected PC3 cells. Cell area (c) and sphericity (d) decreased with anti-microRNAs knockdown of miR-130b or miR-301b, indicating a more fibroblast-like phenotype. e RT-qPCR confirms transcriptional signature associated with promotion of cellular senescence after forced expression of miR-130b or miR-301b. f attenuation of senescent phenotype following inhibition of endogenous levels of each miRNA. h Western blot shows that LMNB1 levels are downregulated when miR-130b or miR-301b are overexpressed, concomitantly with locus-specific H3K9me3 increase. All data are presented as mean of three independent experiments ± s.d. (*p < 0.05, **p < 0.01, ***p < 0.001)