Fig. 6

miR-130b and miR-301b impair DNA-damage signalling pathway. a Comet assay immunofluorescence images of PC3 cells transfected with pre-miRNAs and counterstained with Syber Green, depicting DNA-damage associated morphology. b Graphic representation of parameters analysed in the assay, supporting that both miR-130b and miR-301b overexpression induce DNA damage in PC3 cells. c RT-qPCR evaluation of multiple genes involved in DNA-damage response pathway. All data are presented as mean of three independent experiments ± s.d. (*p < 0.05, **p < 0.01, ***p < 0.001)