Fig. 2

SP1 activates AGAP2-AS1 expression in GC cells. a JASPR online prediction of SP1 binding sites in the AGAP2-AS1 promoter regions. b Western blot analysis of SP1 protein levels in the AGS and BGC823 cells after transfection with SP1 siRNA. c qRT-PCR analysis of SP1 and AGAP2-AS1 expression in AGS and BGC823 cells after transfection with SP1 siRNA. d Western blot and qRT-PCR analyses of SP1 and AGAP2-AS1 expression in the AGS and BGC823 cells after transfection with SP1 vector or empty vector. e ChIP-qPCR analysis of SP1 occupancy in the AGAP2-AS1 promoter regions in the BGC823 and AGS cells. IgG was used as a negative control. f Luciferase reporter analysis of luciferase activity in the HEK293 cells cotransfected with pGL3-AGAP2-AS1 and SP1 vector or an empty vector. *P < 0.05, **P < 0.01