Fig. 6

AGAP2-AS1 knockdown inhibits GC cell tumor growth in vivo. a The BGC823 cells with stable knockdown of AGAP2-AS1 were used for the in vivo tumorigenesis assays. The tumors formed from the BGC823 cells with AGAP2-AS1 knockdown and the control cells in nude mice are shown. b The tumor growth curves were measured 3 days after the injection of the BGC823 cells once the tumor had formed, and the volume was calculated every 3 days. c Tumor weights in the sh-AGAP2-AS1 and control groups are presented. d qRT-PCR analysis of AGAP2-AS1 expression levels in the tumor tissues formed from the AGAP2-AS1-downregulated cells or control cells. e Tumors formed from sh-AGAP2-AS1-transfected BGC823 cells showed lower Ki67-positive signals than tumors formed from the control cells. Upper; hematoxylin & eosin staining, Lower Ki67 immunostaining. *P < 0.05, **P < 0.01