Fig. 2
From: Incorporation of a hinge domain improves the expansion of chimeric antigen receptor T cells
Hinge incorporation mainly promotes CD4+ CAR T cell expansion. a Flow cytometric analysis of the percentage of CD4+ and CD8+ 19-H.28z T (left), Meso-H.28z T (middle), and PSCA-H.28z T (right) during the in vitro culture period. Black box represents CD4+ T cell and gray box represents CD8+ T cells. The data are representative of independent experiments verified with cells from over three individual healthy human donors. b Flow cytometric analysis of the percentage of CD4+ and CD8+ PSCA-H.28z T (left) and MUC1-H.28z T cells (right) during the in vitro culture period. Black box represents CD4+ T cell and gray box represents CD8+ T cells. The data are representative of independent experiments verified with cells from over three individual healthy human donors. c Flow cytometric analysis of the percentage of CD4+ and CD8+ GFP T, 19.28z T, 19-H.28z T, PSCA.28z T, and PSCA-H.28z T cells when CD4+ T and CD8+ T cells were isolated and cultured them separately in vitro. The data are representative of independent experiments verified with cells from over three individual healthy human donors