Fig. 6

PS inhibits pericellular FN polymerization via triggering AKT phosphorylation which reduces ERK phosphorylation. a IBs were probed for AKT phosphorylation, ERK phosphorylation, polyFN, and monoFN in lysates of suspended LLC cells treated without or with 100 μM of PS, 10 μM of PI3K inhibitor LY294002 (LY) or mixture of both for 4 h in suspension. Quantifications of pAKT (b), pERK (c), polyFN (d), and of monoFN (e) that were normalized by GAPDH levels in the IBs in (a). f Representative images of IF staining for the polyFN assemblies on suspended LLC cells transfected with Scr or shAKT#1 and 2 in the absence or presence of 100 μM of PS for 4 h. g Quantifications of polyFN levels on LLC cells as the treatment in (f). h The same IBs as in (a) for lysates of suspended LLC cells transfected with Scr shRNA or shAKT#1 in the absence or presence of 100 μm of PS for 4 h. Quantifications of AKT (i), pAKT (j), ERK (k), and pERK (l) were normalized by GAPDH levels in the IBs in (h)