Fig. 7

The activated PI3K/AKT-induced ERK inactivation mediates the inhibitory effect of PS on polyFN polymerization on and mouse lung metastasis of suspended LLC cells. a IBs were probed for total ERK, pERK, and GAPDH in lysates of suspended LLC cells treated without or with 100 μM of PS, PS + 20 μM of PI3K inhibitor LY or PS + LY+ 20 μM of ERK inhibitor U0126 for 4 h in suspension. Note: Images of the same proteins were trimmed from the same IB gels. b Quantifications of pERK that was normalized by GAPDH levels in the IBs in (a). c Representative images of IF staining for the polyFN assemblies on suspended LLC cells that were treated similarly as described in (a). The positions of tumor cells were circled with white dashed lines. d Quantifications of polyFN levels on LLC cells that were treated as in (c). e Mouse lungs (upper panel) were taken, and tumor nodule numbers (lower panel) on lung surfaces from mice bearing the intravenously inoculated LLC cells that were treated with DMSO, PS, PS + LY or PS + LY + U0126 as described in (a) were counted upon mouse sacrifices