Fig. 6

ELF2 isoform expression affects early lymphocytic development. Analysis of ELF2⁺ (GFP ⁺) retrogenic ‘double negative’ (DN), ‘double positive’ (DP) or single positive CD4⁺ and CD8⁺ T cells (a), and DN cells at each developmental stage in the thymus (b); representative flow cytometry plots indicating perturbation of the T cell compartment are shown (a–b). Analysis of ELF2⁺ (GFP ⁺ ) retrogenic B cells in the bone marrow stained with CD43 and B220⁺ (c) and with IgM and B220⁺ (d). Analysis of B cell maturation in the spleen (e), and myeloid subpopulations in bone marrow (BM) and spleen (f), of ELF2⁺ retrogenic mice. Data represents the mean ± SEM of three experiments each performed with 4–5 mice per experimental arm. Statistical analysis performed using Student’s t test (ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001)