Fig. 2

LncRNA uc.134 suppresses the proliferation, migration, and invasion of HCC in vitro and in vivo. a Growth curves of indicated HCC cells that overexpressed uc.134 compared with those of negative control cells and untransfected cells were determined with a CCK8 proliferation analysis. The mean ± SD is shown for five independent experiments. ***P < 0.001. b Representative images of colony formation assays (left panels); analysis of the number of colonies (right panels). All experiments were performed in triplicate, and results are presented as mean ± SD. ***P < 0.001. c Invasion ability of the indicated cell lines by Transwell assays. All experiments were performed in triplicate, and results are presented as mean ± SD. ***P < 0.001. d Representative images of the scratch wound-healing assay. The wound-healing percentage was analyzed with the ImageJ software. All experiments were performed in triplicate, and results are presented as mean ± SD. **P < 0.01 and ***P < 0.001. e Representative images of tumors formed in nude mice (n = 6). *P < 0.05. f Lung metastasis model generated by injecting tumor cells into the tail veins of mice. HE staining showing the number and volume of lung metastases in each group (n = 6); **P < 0. 01 and ***P < 0.001