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Table 2 Gene-specific primers SZ-123VH and SZ-123VK

From: The chimeric monoclonal antibody MHCSZ-123 against human von Willebrand factor A3 domain inhibits high-shear arterial thrombosis in a Rhesus monkey model

p-VH-F:

GCGAATTCCACCATGGAGACAGACACACTCCTGCTATGGGTACTG

CTGCTCTGGGTTCCAGGTTCCACTGGTCAGGTCCAACTGCAGCAG

CCTGGGGCTGAACTGGTGAAG

p-VH-R:

AGTGGCGGCCGCGGATCCGAATTTTAAGTCATTTA

p-VK-F:

GCGAATTCCACCATGGAGACAGACACACTCCTGCTATGGGTACTGC

p-VK-R:

TGGATCAGTTATCTATGCGGCCGCTAACACTCT

  1. The underlined bases represent the recognition sites for the restriction enzymes EcoRI and NotI