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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Intrathymic injection of hematopoietic progenitor cells establishes functional T cell development in a mouse model of severe combined immunodeficiency

Fig. 2

Intrathymic injection of hematopoietic stem/progenitor cells establishes thymopoiesis in young and aged NSG mice. a NSG mice were intrathymically injected with 10,000 C57BL/6 LSK cells. Thymuses were harvested on day 56 and analyzed by flow cytometry. Left panel shows thymocyte subsets for untreated NOD mice included as a reference group. Center and right panels show frequency and absolute numbers for thymocyte subsets of injected NSG mice. Data combined from two independent experiments are shown. Mean and SEM are presented (n = 8–12). b NSG mice were treated as in (a). Spleens were harvested on day 56 and T cells were analyzed by flow cytometry. Combined data from two independent experiments are shown. Mean and SEM are presented (n = 8–10). c Mice were treated as in (a). Thymuses and spleens were harvested 5 months after injection and analyzed by flow cytometry. Left panel shows frequency of thymocyte subsets; center panel shows absolute numbers of thymocyte subsets, and right panel shows absolute numbers of splenic T cells. Data combined from two independent experiments are shown. Mean and SEM are presented (n = 8–12). d Mice were treated as in (a) for aged mice (6 months old) and young mice (6 weeks old). Thymuses and spleens were harvested at day 56. Panels 1 and 2: absolute numbers of thymocytes and DP thymocytes in young versus aged mice. Panels 3 and 4: absolute numbers of CD4+ and CD8+ splenic T cells in young versus aged mice. Data combined from two independent experiments are shown. Mean and SEM are presented (n = 8–12)

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