Fig. 5

SIX3 reduces numerical centrosomal aberrations. U251 cells stably expressing SIX3 (U251-SIX3) and U251 cells stably expressing GFP (U251-CON) were constructed using lenti-virus. U251-CON and U251-SIX3 cells were synchronized with double thymidine block and blocked at M phase with MG132 (20 μM) for 2 h. a Immunofluorescence analysis showed typical multi-centrosomes and chromosome unalignment (white arrows) in U251-CON and U251-SIX3 cells (scale bars, 20 μm). b Centrosomes in more than 200 cells were analyzed. c More than 100 cells were observed for unaligned chromosomes