Fig. 4
AD0157 impairs corneal neovascularization in mice. a Representative flat-mounted cauterized corneas harvested from control and AD0157-treated mice at day 9 post-injury. Lymphatic (LYVE-1 positive) and blood (CD31 positive) vessels appear in green and in red, respectively, at low (left panels) and high (middle panels) magnifications. Scale bars represent 1000 and 500 μm, respectively. Panels at right correspond to representative pictures of filopodia-like structures (white arrowheads) displayed by migrating LECs. Scale bars represent 10 μm. b Computerized measurements of different parameters: lymphatic area density (area covered by neoformed lymphatic vessels), length density (cumulative length of vessels), branching density (number of bifurcations), end-point density (number of sprout tips), spatial lymphatic distribution (a grid was applied on each cornea picture to establish the distribution curves of capillaries around the limbal vessels; Ni corresponds to the number of vessel intersections with the grid versus the distance to the limbus), number and length of filopodia-like structures in a total length of 25 μm at the end of the lymphatic vessel, and blood area density (area covered by neoformed blood vessels). Results were divided by the total cornea area to obtain densities. Values are expressed as mean ± s.e.m., and the Wilcoxon-Mann-Whitney significance tests were used to compare the differences between control and AD0157 treatments.*p < 0.05, **p < 0.01, ***p < 0.001 (n = 12 mice/group)