Fig. 2

In vitro assays to compare the numbers and potencies of HSPCs between the Asxl1 G643WfsX12 heterozygous mice and wild type controls. a Cobblestone-area-forming cell assays on bone marrow cells from Asxl1 ^tm/+ mice showed more colonies than those from wild-type control mice (N = 3 each). b Colony-forming cell assays and serial plating assays showed no significant difference in the ability of forming colonies and differentiation into different lineages in the first plating between Asxl1 ^tm/+ bone marrow cells and wild controls. However, in the second plating Asxl1 ^tm/+ cells formed more colonies than wild-type controls. This trend lasted to the third plating but only two wild-type samples had sufficient cells for the third plating, so we were unable to perform t test in the third plating (first plating: wild-type N = 5, Asxl1 ^tm/+ N = 7; second plating: wild-type N = 5, Asxl1 ^tm/+ N = 6; third plating: wild-type N = 2, Asxl1 ^tm/+ N = 6). *p < 0.05