Fig. 1
Expression and activation status of EPH-A2, EPH-B and related Ephrin ligands in ERMS tumours and cell lines. a EPH-A2 and b Ephrin-A1 transcript levels in 14 ERMS primary tumours and NSM, as measured by Q-PCR assays. GAPDH mRNA was used as an endogenous control. The relative mRNA expression levels are presented as the average fold changes (RQ) in tumour biopsies vs. NSM, set at 1. Error bars represent the RQmax and RQmin values of at least two independent assays, each performed in triplicate. c Western blots showing the expression levels of EPH-A2, EPH-B, Ephrin-A1 and Ephrin-B2 proteins as well as the phosphorylation status of EPH-A2 (p-EPH-A2) and EPH-B (p-EPH-B) in RD and TE671 cell lines in comparison to NSM; α-Tubulin was used as loading control. Representative images of three different experiments