Fig. 4
From: Kinase profiling of liposarcomas using RNAi and drug screening assays identified druggable targets
Ponatinib treatment inhibits KIT signaling and clonogenic growth of liposarcoma cells. a Ponatinib (250–1000 nM, 16 h)-treated liposarcoma cell lines LPS141 (well-differentiated) and MLS402 (myxoid) were analyzed for KIT signaling pathway by western blotting. β-actin and GAPDH served as loading controls. b Anchorage-dependent colony formation assay of LPS141 and MLS402 cells treated with either ponatinib (250–1000 nM, 10 days) or diluent control. Colonies were stained with crystal violet (microscope images in left panel) and quantification of staining intensity is shown by bar graphs (right panel). c Soft agar colony formation (anchorage-independent) of LPS141 and MLS402 cells cultured with either ponatinib (250–1000 nM, 21 days) or diluent control. Number of colonies shown as bar graphs. Experiments were done in triplicates and repeated 3 times. *P value ≤ 0.01